Journal: Journal of Extracellular Vesicles

Article Title: Sublytic Activity of a Pore‐Forming Protein From Commensal Bacteria Causes Epigenetic Modulation of Tumour‐Affiliated Protein Expression

doi: 10.1002/jev2.70149

Figure Lengend Snippet: Intratumoural administration of ClyA + OMVs is therapeutically active against a colon cancer mouse model. (a) Schematic illustration of how a colon cancer murine model was established by subcutaneous inoculation of the rapidly proliferating CT26 cell line in ATHYM‐ Foxn1 nu mice. The mice were randomly distributed into two groups (five mice per group) after 10 days of postinoculation. Mice were repeatedly injected intratumorally on Days 11, 13, 15 and 17 with ClyA + OMVs (10 µg/dose) or ClyA − OMVs (10 µg/dose) and subsequently sacrificed for analyses on Day 21. (b, c) Representative image and histogram showing that ClyA + OMVs significantly caused a reduction in the tumour volume compared to the ClyA − OMV‐treated animal group. Data is presented as mean ± s.d. Statistical significance was assessed using a non‐parametric t ‐test, whereby * p ≤ 0.05. Scale bar = 1 cm. (d) Representative H&E‐stained images showing reduction of tumour size in the ClyA + OMVs treated animals, compare to animals treated with ClyA − OMVs. Scale bar = 500 µm. Insets for different treatment conditions is presented on the right side of each figure panel. Yellow arrowheads indicate necrotic areas. Scale bar = 20 µm.

Article Snippet: A colon cancer mouse tumour model was established using ATHYM‐ Foxn1 nu (Foxn1 nu athymic nude) mice (purchased from Janvier Labs, France) and CT26 mouse colon cancer cell line.

Techniques: Injection, Staining